Archive for October, 2013

DNA transformation with friends

last Thursday we performed the first set of DNA transformations with a small circle of friends, Ferenc Szalai, Attila Nemes and András Márton Juhász. this was a basic test of the lab to prove if multiple people can work in it together.

our lab featured at dyibio.org

diybio-logo-black-90x60pxOur lab just got featured at dyibio.org! find is in the local group listings 🙂

first successful DNA transformation in the new lab

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Yesterday I performed the first successful DNA transformation in the new lab, set up at FabLab Budapest. The transformation was simply the pGLO gene earlier isolated at the University of Szeged, into e.coli HB 101. The green spots on the image above are the bacteria with the Green Fluorescent Protein in then.

This gives reassurance that the crude tools here in the lab can do real work 🙂

I’ve also summed up most of the basic protocols, ways to grow bacteria and the DNA transformation steps as well – because I always forget everything.