first successful DNA transformation in the new lab
- Posted by darkeye on October 1st, 2013 filed in experiments
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Yesterday I performed the first successful DNA transformation in the new lab, set up at FabLab Budapest. The transformation was simply the pGLO gene earlier isolated at the University of Szeged, into e.coli HB 101. The green spots on the image above are the bacteria with the Green Fluorescent Protein in then.
This gives reassurance that the crude tools here in the lab can do real work 🙂
I’ve also summed up most of the basic protocols, ways to grow bacteria and the DNA transformation steps as well – because I always forget everything.
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