bio.display

For the experiments done at the Ars Electronica Center, I’m using 12x12cm plates again. This means that each plate would take about 120mm x 120mm x 7mm = 100800mm³ = 100.8ml. This means I can pour about 10 plates / liter of LB agar with bacteria in there. As per my protocol page, each liter of LB agar with bacteria will need 55ml of overnight liquid culutre. Thus, if my aim is to create 40 plates, I need to prepare for 4 liters of LB agar with bacteria, which is 55ml x 4 = 220ml of liquid cutlure.

As they have 500ml Erlenmayer flasks here, of which a quater volume (125ml) can be used for the liquid culture, I need to use two of them, which will bring me to 250ml of liquid culture. To create the same amount again based on liquid cultures, I’ll need 2ml of liquid culture to mix with fresh LB medium.

For a 125ml of liquid culture, I’ll need:

• 125ml LB medium
• 6250μg = 6.25mg of ampicillin (a final concentration of 50μg/ml)
• some bacteria from the streaked out plate

I’ll make two of these.

I’ll also make a smaller, 100ml Erlenmayer flask control with arabinose – to see if it glows as expected. This will contain:

• 25ml LB medium
• 1250μg = 1.25mg of ampicillin (a final concentration of 50μg/ml)
• some bacteria from the streaked out plate
• 125mg or arabinose (a final concentration of 5mg/ml)