bio.display

As we’ll need to do a lot of DNA transformation using the gene generating the pGLO GFP, we have to extract some from bacteria that we grow. The idea is to grow lots of bacteria, then use a DNA purification kit to separate the DNA plasmid from the bacteria itself. Of course, all the bacteria die a gruesome death at 20.000 g acceleration in the process – but not in vain.

We’re going to use a Qiagen Plasmid Midi Kit for this purpose next Thursday. Fortunately the protocol and all details are available online – it’s quite a tedious process…